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tissuelyser lt  (Qiagen)


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  • 99

    Structured Review

    Qiagen tissuelyser lt
    Tissuelyser Lt, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3804 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stainless+steel+beads/Stainless+Steel+Beads/pm42869257-290-19-21
    Average 99 stars, based on 3804 article reviews
    tissuelyser lt - by Bioz Stars, 2026-10
    99/100 stars

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    Centrifugation:

    Article Title: Turning urban wildlife mortality into a surveillance tool: Detection of vector-borne pathogens in carcasses of hedgehogs, squirrels, and blackbirds
    Article Snippet: .. Samples were homogenized (30% w / v ) in RLT buffer (Qiagen, Hilden, Germany) with β-mercaptoethanol, using stainless steel beads in a TissueLyzer II (Qiagen, Hilden, Germany), followed by digestion with proteinase K. After centrifugation, RNA and DNA were extracted from the supernatants using the QIAamp Viral RNA Mini Kit and DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) respectively, according to the manufacturer's instructions. ..

    RNA Sequencing:

    Article Title: IGS38, a lncRNA from the human rDNA intergenic spacer, regulates rRNA transcription by altering rDNA chromatin organisation and activating the transcription machinery
    Article Snippet: 5μg/μl of the samples were loaded onto an Agilent high-sensitivity RNA 6000 Pico-Chip and analysed in a 2100 Bioanalyzer system. .. Small RNA-seq was performed by lysing freshly frozen HeLa cells with Tissue Lyser LT (Qiagen) with 5mm Stainless Steel Beads (Qiagen) in Qiazol (Qiagen). .. RNA was extracted with miRNeasy Micro kit (Qiagen).

    Incubation:

    Article Title: Therapy for colorectal and small intestine cancers
    Article Snippet: Untargeted Metabolites Profiling The excised animal tissues or tumors were added to 2 mL Eppendorf tubes containing 600 μL of 3 mM monobromobimane (MBB) in CH3OH:H2O (80:20) at −20° C. and incubated for 2 h, followed by 1 h incubation at 0° C. Here, MBB was used to react with thiols and protect them for further oxidation. .. This initial incubation was followed by tissue disruption using stainless steel beads in a TissueLyser (Qiagen) and an additional 30 min at −20° C. Extracts were centrifuged for 15 min at 13000 rpm to pellet insoluble material and supernatants were transferred to clean tubes. ..

    Disruption:

    Article Title: Therapy for colorectal and small intestine cancers
    Article Snippet: Untargeted Metabolites Profiling The excised animal tissues or tumors were added to 2 mL Eppendorf tubes containing 600 μL of 3 mM monobromobimane (MBB) in CH3OH:H2O (80:20) at −20° C. and incubated for 2 h, followed by 1 h incubation at 0° C. Here, MBB was used to react with thiols and protect them for further oxidation. .. This initial incubation was followed by tissue disruption using stainless steel beads in a TissueLyser (Qiagen) and an additional 30 min at −20° C. Extracts were centrifuged for 15 min at 13000 rpm to pellet insoluble material and supernatants were transferred to clean tubes. ..

    Tissue:

    Article Title: Functional Improvement of dystrophic muscle by utrophin gene editing
    Article Snippet: 389 390 Protein extraction and Western blotting 391 Cells and tissues were lysed in ice-cold RIPA buffer (#9806, Cell Signaling Technology) 392 supplemented with cOmplete Mini protease inhibitor cocktail (#11836153001, Roche) and 1 393 mM phenylmethylsulfonyl fluoride (PMSF, #52332-5GM, Sigma-Aldrich). .. For tissue samples, 5 394 mm stainless steel beads (#69989, Qiagen) were added to 1× RIPA buffer, and homogenization 395 was performed using a TissueLyser LT (#85600, Qiagen) at 30 cycles s−1 for 2 min. Lysates were 396 centrifuged at 14,000 × g for 15 min at 4 °C, and supernatants collected. .. Protein concentrations 397 were determined using the Pierce BCA Protein Assay Kit (#23227, Thermo Fisher Scientific) 398 according to the manufacturer’s instructions.

    Homogenization:

    Article Title: Functional Improvement of dystrophic muscle by utrophin gene editing
    Article Snippet: 389 390 Protein extraction and Western blotting 391 Cells and tissues were lysed in ice-cold RIPA buffer (#9806, Cell Signaling Technology) 392 supplemented with cOmplete Mini protease inhibitor cocktail (#11836153001, Roche) and 1 393 mM phenylmethylsulfonyl fluoride (PMSF, #52332-5GM, Sigma-Aldrich). .. For tissue samples, 5 394 mm stainless steel beads (#69989, Qiagen) were added to 1× RIPA buffer, and homogenization 395 was performed using a TissueLyser LT (#85600, Qiagen) at 30 cycles s−1 for 2 min. Lysates were 396 centrifuged at 14,000 × g for 15 min at 4 °C, and supernatants collected. .. Protein concentrations 397 were determined using the Pierce BCA Protein Assay Kit (#23227, Thermo Fisher Scientific) 398 according to the manufacturer’s instructions.



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